Mut3a. Posted in Mut3a um 2:23 pm von jasir12. Mutah Mutah ist Arabisch und bedeutet Vergnügen. Schiiten verstehen darunter eine Ehe auf Zeit. Ein Mann bezahlt eine Frau (mit einer sogenannten Mitgift) und kann dann eine sexuelle Beziehung mit ihr haben und zwar so lange wie es im Mutah-Vertrag (Ehevertrag) vorher festgelegt wurde. Die Mutah-Ehe kann weniger als eine. die Mutha ist eine Zeitehe die Shia ist die Glaubensrichtung der Schiiten die Sunna ist die Glaubensrichtung der Sunniten Der Streit zwischen Schiiten und Sunniten geht jetzt so in etwa seit 1400 Jahren
mut3a. Hallo. Ich habe eine Freundin, die ist Schiitin. Nun habe ich einen großen Streit von ihrer Freundin mit einen verrückten Stalker mitbekommen, der sie bedroht. Dabei fielen Begriffe, die ich nicht verstanden haben. Natürlich könnte ich meine Freundin fragen, aber ich hätte gerne eine neutrale Sicht der Dinge. Es handelt sich um folgende Begriffe: - mut3a(-Kinder) - Shia - Sunna. Mut'aa marriage Interview. Just to let the people see how it is really done Nikah mut'ah Arabic: نكاح المتعة , romanized: nikāḥ al-mutʿah, literally pleasure marriage;: 1045 or Sigheh (Persian: صیغه ) is a private and verbal temporary marriage contract that is practiced in Twelver Shia Islam in which the duration of the marriage and the mahr must be specified and agreed upon in advance.: 242: 47-53 It is a private contract made in a verbal.
. This 'marriage of mut'a' is referred to both in the hadith literature and, in much more detail, in the books on jurisprudence (fiqh). In the hadith and in other sayings related from early Muslims the word 'mut'a' itself is usually employed Liebe Brüder und Schwestern im Islam! Wir haben diesen Blog eröffnet, um zu versuchen, unseren muslimischen Geschwistern die Wahrheit offen zu legen, das Gute zu gebieten und das Schlechte zu verwehren.. Jazakamullah Khairan Möge Allah subhana wa ta3ala uns Geduld und Standhaftigkeit schenken The GFP gene is GFP mut3a from Cormack et al (Gene 173:33-38) and contains engineered ribosome binding site. DNA inserts can be introduced at any of the polylinker sites upstream of the XbaI site. Promoter activity can be monitored by measuring GFP synthesis by fluorescence microscopy, fluorimetry or flow cytometry. How to cite this plasmid ( Back to top) These plasmids were created by your.
Es überliefert der (für die Rafidah) Vertrauenswürdige: Die Muta (Zeitehe) ist meine Religion und die Religion meiner Väter, wer also die Muta ausübt, der hat unsere Religion ausgeübt und wer sie ablehnt, der hat unsere Religion abgelehnt und praktiziert eine andere Religion, als die unsere Da7ik Wa Mut3a. 3.2K likes. Pour s'amuser et rire. Facebook is showing information to help you better understand the purpose of a Page This article was co-authored by our trained team of editors and researchers who validated it for accuracy and comprehensiveness. wikiHow's Content Management Team carefully monitors the work from our editorial staff to ensure that each article is backed by trusted research and meets our high quality standards. There are 15 references cited in this article, which can be found at the bottom of.
gyrA mutto roe gyrA MUT3A) gyrA mutto roe gyrA MUT3B) gyrA mutto roe gyrA MUT3C) gyrA mutto roe gyrA MUT3D) rrs ocus otrol rrs rrs l te roe rrs WT1) rrs l te roe rrs WT2) rrs mutto roe rrs MUT1) rrs mutto roe rrs MUT2) embB ocus otrol embB embB l te roe embB WT1) embB mutto roe embB MUT1A) embB mutto roe embB MUT1B) coloure mrer oute otrol mlfcto otrol M. tuberculosis comle gyrA ocus otrol. In Islam the word most commonly employed for marriage is nikah, which means literally 'sexual intercourse'. As a legal term it denotes the situation resulting from a particular contract, entered into by a man and a woman, by which sexual intercourse between them becomes legitimate in the eyes of God and society. The only other mode of legitimizing this sexual relationship i The equilibrium process of six mutants (mut2N, mut2C, mut3a, mut3a, mut6G, and mut6G) was performed with the similar procedure as wild-type ubiquitin. SMD simulations with constant loading force were performed to wild-type ubiquitin, mut2N and mut2C. The C α atoms of the terminal residues of protein were subjected to constant pulling forces of 667 pN or 800 pN in the z direction. One hundred. Mut3a wife? Hello: she is a prostitute! But I have a few questions for you our dear khurram the wannabe Arab. 1. How much would you pay for a mut3a marriage aka Islamic prostitution? Oh did I call is prostitution? 2. And how much would the mullah get for a fee from the man and the woman? oh did I call him Mullah? 3. And is the mullah here is. Diese Webseite verwendet Cookies. Mit einem Klick auf Zustimmen akzeptieren Sie die Verwendung der Cookies. Die Daten, die durch die Cookies entstehen, werden für nicht personalisierte Analysen genutzt.Weitere Informationen finden Sie in den Einstellungen sowie in unseren Datenschutzhinweisen.Sie können die Verwendung von Cookies jederzeit über Ihre Einstellungen anpassen
How Do I Do Mutah? The Mutah Marriage Contract Procedure . Do Your Research . Firstly we would advise you to get acquainted with the background of Mutah and its Islamic legitimacy Mut3a-Ehe! Antworten: Das sind doch aber keine Orfi-Ehen oder Zeitehen, wenn zumindest eine Partei davon ausgeht, dass sie rechtmäßig verheiratet ist. Schiitische Zeitehen zeichen sich ja gerade dadurch aus, dass beide Parteien wissen, dass ein Vertrag auf Zeit geschlossen wird und Orfi-Ehen in Ägypten werden vor allem mit Touristinnen geschlossen. Der Unterschied hier ist doch wirklich. After feeding shrimp three times daily in 400‐L cement tanks for 9 weeks, no significant differences in the average shrimp weight, the number of BS11 in either the culture water or in the shrimp's gut were seen between shrimp fed BS11‐GFP and BS11‐pAD or BS11, suggesting that expression of the gfpmut3a gene has no detectable effect on BS11 properties and shrimp growth. Histological. naseeb.com - muslim social network WT1 WT2 WT3 MUT1 MUT2 MUT3A MUT3B MUT3C MUT3D rrs rrs rrs rrs rrs WT1 WT2 MUT1 MUT2 CC AC TUB embB embB embB embB WT1 MUT1A MUT1B # DD.MM.YY 01234 N E H R E Geno MTBDRsType l 96 VER 1.0 00317-0613-03-3 LOT HYB min min minSTR SUB dd mm yyyy M M TUB gyrA WT rrs WT rrs MUT gyrA MUT FLQ B WT B MUT e tant e tant e tant AG/ CP EMB CC AC # DD.MM.YY 01234 N EH R Geno MTBDRsType l 12 VER 2.0 00317A.
The GFP gene is GFP mut3a from Cormack et al (Gene 173:33-38) and contains engineered ribosome binding site. DNA inserts can be introduced at any of the polylinker sites upstream of the XbaI site. Promoter activity can be monitored by measuring GFP synthesis by fluorescence microscopy, fluorimetry or flow cytometry. How to cite this plasmid ( Back to top) These plasmids were created by your. Indeed, if mut3A and Δ3 were to result only in defects in the interaction between nsP1 and nsP4, it would be logical to assume that compensatory mutations could take place in either of the proteins. However, we were unable to find any mutations in the nsP4 region either in the plaque-purified viruses described above or in a larger number of isolates, purified and analysed specifically for.
Da7ik Wa Mut3a. 3.2K likes. Pour s'amuser et rir 16322 Ensembl ENSG00000123999 ENSMUSG00000032968 UniProt P05111 Q04997 RefSeq (mRNA) NM_002191 NM_010564 NM_001329843 RefSeq (protein) NP_002182 NP_001316772 NP_034694 Location (UCSC) Chr 2: 219.57 - 219.58 Mb Chr 1: 75.51 - 75.51 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Inhibin, alpha, also known as INHA, is a protein which in humans is encoded by the INHA gene. Contents.
Literal meaning of the Arabic word 'Muta' is 'enjoyment'; therefore, it may also be regarded as 'marriage for pleasure'. In the earlier days of Islam, when the Arabs had to live away from their homes for a considerably long period either on account of wars or on trade-journeys, they used to satisfy their sex-desires through prostitutes بالدليل من كتب الشيعة والسنة تُثبت أنّ زواج المتعة حرام ومعممي الشيعة يُزوّرون الحقيقة..
inhA mutation probe 3A (inhA MUT3A) inhA mutation probe 3B (inhA MUT3B) Coloured marker -100 0 100 200 -dF/dT Targeted (amplicon) sequencing Read alignment Whole genome sequencing M tuberculosis DNA ampliﬁcation Sequencing results M tuberculosis Lineage 2 Beijing Resistance proﬁle RIF INH PZA EMB FLQ KAN AMK CPM ETH LZD BDQ CFZ Mutation library Conjugate control Ampliﬁcation control. WT1 WT2 MUT3A MUT3B rpoB WT1 WT2 WT3 WT4 WT5 WT6 WT7 WT8 MUT1 MUT2A MUT2B MUT3 rpoB rpoB rpoB rpoB rpoB rpoB rpoB rpoB rpoB rpoB rpoB katG WT MUT1 MUT2 katG katG katG inhA WT1 WT2 MUT1 MUT2 MUT3A MUT3B inhA inhA inhA inhA inhA inhA # DD.MM.YY 01234 N E H R E Geno MTBDRType® plus 12 VER 1.0 00304-0507-02-2 LOT HYB min min minSTR SUB dd mm yyyy M CC AC TUB M TUB rpoB-WT katG-WT -MUT rpoB-MUT. Bacillus anthracis , the causative agent of anthrax, is known for its rapid proliferation and dissemination in mammalian hosts. In contrast, little information exists regarding the lifestyle of this important pathogen outside of the host. Considering that Bacillus species, including close relatives of B. anthracis , are saprophytic soil organisms, we investigated the capacity of B. anthracis.
MUT3A (T8C): present absent MUT3B (T8A): present absent Second line LPA gyrA:--locus control: present absent WT1 (85-90): present absent WT2 (89-93): present absent WT3 (92-97): present absent MUT1 (A90V): present absent MUT2 (S91P): present absent MUT3A (D94A): present absent MUT3B (D94N/Y): present absent MUT3C (D94G): present absent MUT3D (D94H): present absent gyrB:----locus control. Four types of double mutations gyrA MUT3A and MUT3C, gyrA MUT3B and MUT3D, gyrA MUT3B and MUT3C, and gyrA MUT1 and MUT3A were found in 8/324 (2.4%) isolates. A total of 26 isolates showed missing of wt probe only, which includes 20, 4, and 2 in WT 3-, WT 2-, and WT 1-2-, respectively. Ten isolates (10/324, 3.1%) missing gyrB WT1- showed a low prevalence of gyrB mutations in this region. For.
Mut3A or Δ3 mutations eliminate the ability of nsP1 to induce the formation of such structures; however, the compensatory mutations restore the formation of these structures at 37 and 28 °C (data not shown) even though they do not repair palmitoylation. The biological significance of these effects for SFV infection is not known. This phenomenon, however, clearly correlates with the strong. mut3A) was subcloned into pAS2-1 for expression of the mutant peptide as Gal4-binding domain (BD) fusion-protein. NuMA aa 1788-1888 (NuMA-ΔC) with tandem alanine substitutions, I1800A, I1801A, N1802A, I1803A, T1804A, and M1805A (NuMA- ΔC mut6A) were subcloned into pACT2 for expression of the mutant peptides as Gal4-activation domain (AD) fusion-protein. Protein 4.1R exon 20-21 deletion. Free Mutah Matchmaking Service . We have decided that while this site is under construction we would still like to provide a basic platform for Muslim males and females (and females who are Christian, Jewish, Zoroastrian, or believers in One God) to be able to search for a Mutah spouse
jihad al nikah or (pleasure marriage)... to marry the terrorists in a bid help them better fight against the Syrian government forces. The insurgency in Syria, which is revealing horrifying aspects from shooting and killing women and children to the cannibal act of eating organs of a Syrian soldier by terrorists, has now turned to a serious concern for families whose daughters are being. The MTBDRsl assay (Hain Lifescience GmbH, Germany) is a new line probe assay for the detection of extensively drug-resistant tuberculosis (XDR TB). The test simultaneously detects resistance to ethambutol, aminoglycosides/cyclic peptides, and fluoroquinolones through detection of mutations in the relevant genes. The assay format is identical to the MTBDR Hain assay 1. Introduction. The study of gene expression in Bacillus cereus is a largely unexplored area of biology although B. cereus has diverse biological activities in the environment.B. cereus is ubiquitous in soil and on plant roots (Stabb et al., 1994), and certain strains of B. cereus produce antibiotics (Wakayama et al., 1984; Kamogashira et al., 1988; Tschen and Tseng, 1989; Silo-Suh et al. In conditions of halted or limited genome replication, like those experienced in sporulating cells of Bacillus subtilis, a more immediate detriment caused by DNA damage is altering the transcriptional programme that drives this developmental process. Here, we report that mfd, which encodes a conserved bacterial protein that mediates transcription‐coupled DNA repair (TCR), is expressed.
Primers specifically amplify a 732 bp fragment of the gfpmut3a gene. Both wild‐type strains lack the corresponding genetic information. Expression of GFP could be observed in both genotypes during all stages of growth. Control cells lacking pAD43‐25 showed no fluorescence. Representative results for the log phase are shown. Bars represent 100 μm (ERIC I) and 10 μm (ERIC II) The failure of GFP‐4.1R 24 kD mut3A to colocalize with NuMA in intact cells suggests that V762, V765, and V767 of 4.1 R are necessary for association with NuMA, and are consistent with our results from yeast two‐hybrid and in vitro binding assays This is a ColE1-mobilizable plasmid, containing the mut3a-gfp gene encoding a Green Fluorescent Protein expressed under a heterologous promoter, and an nptII kanamycin resistance gene. In order to. Ruling 2542. In a revocable divorce, a man can return to his wife in two ways: 1. he says something that means he has re-established the marriage with her; 2. he does something with the intention of returning to her. Having sexual intercourse ascertains this even if he does not have the intention of returning to her
Concurrent KatG S315 T1 and inhA promoter mutations (T8C (MUT3A)) were detected in only one isolate- an MDR-TB isolate. Table 4 Pattern of gene mutations detected by the MTBDRplus assay in 60 drug-resistant Mycobacterium tuberculosis isolates. Full size table. Discussion. This study reports the accuracy of the Genotype MTBDRplus assay for diagnosing MDR-TB in new and previously treated PTB. The four mutations probes for the inhA gene detected mutations at the codons C15T (inhA MUT1), A16G (inhA MUT2), T8C (inhA MUT3A) and T8A (inhA MUT3B). Frequency of mutations in rifampicin and isoniazid resistant isolates of M. tuberculosis, an analysis from Central India. Frequency of polymorphisms in katG, inhA and rpoB genes and their association with drug resistance. Detection of multidrug.
Even in Iraq it's exaggerated (if we're talking about the common people). Don't you just hate them so much?? They should all go back to Iran!!!1!!!! In Lebanon, like in most of the Arab world, sex is a taboo, and misconceptions about the forbidden topic abound. Even though Lebanon is seen as a bastion of sexual freedom in the Middle East, a lack of sex education, as well as the prevalence of conservative religious and social norms, means that misconceptions about sex run rampant <?abstract?> Die vorliegende Erfindung betrifft ein Verfahren zur enzymatischen Isomerisierung von (R)-3-Hydroxycarbonsäuren zu den entsprechenden 2-Hydroxy-2-methylcarbonsäuren. Die Umw pSMAD3-mut3A-transfected cells (red curve, tracing C) and RAC1B siRNA + vector-transfected cells (green curve, tracing B) are signiﬁcant at 3:00 and all later time points ( p < 0.05, Mann.
Reference of mut3a for shia is at surah nisa 4:24 where they messed up translation for their own need. Reference of 12 imams in Quran does not make any sense, some of them talk about 12 tribes who followed Musa after they migrated out of egypt. I did not understand what it got to do with 12 imams of shia s.o.s 16 August 2017, 17:26 1 1. So does this mean that my neighbors Ali, Hassan or Hussein can no longer rape their 11 and 15 year old sister Zaynab and Zeina and contract a mut3a marriage to cover up the dirty business
Get in touch with لبيك يا نصرالله (@alimadad) — 16 answers, 44 likes. Ask anything you want to learn about لبيك يا نصرالله by getting answers on ASKfm Table 1. Results of isoniazid resistance mutations detected by MTBDRplus test among 227 TB patients with phenotypic resistance to isoniazid in Vietna It is a ColE1- mobilizable plasmid, which bears the mut3a-green fluorescent protein (gfp) gene encoding for a gfp (Cormack et al., 1996) expressed under the E. coli rrnb promoter, and an nptII kanamycin resistance gene. In order to support homologous recombination, a 1000-1300 bp fragment of the corresponding bla genes for each strain was amplified using genomic DNA as a template, and were. One (1/53; 1.9%) isolate harbored two substitutions, i.e., C-15T and T-8C (MUT1 and MUT3A, with all WT bands present). All but one isolates with mutations in the inhA promoter had their katG alleles unreactive to mutant probes. GenoType MTBDRplus identified 42 (42/53; 79.2%) isolates as RIF-resistant. The most prevalent mutation pattern was S531L (MUT3) found in 34 (34/53; 64.1%) isolates.
A gfp (mut3a) gene together with its promoter in a 4.4-kb DNA fragment was obtained from plasmid pGFP4412 by digestion with EcoRI and HindlII . Then the 4.4-kb DNA fragment was ligated to the shuttle vector pHY300PLK digested with HindIII and EcoRI restriction enzymes, thus yielding the plasmid pGFP300 which carries a gfp (mut3a) gen In Mut3a, one must pay the set amount of money for a period of time (can be as little as thirty minutes or more than thirty years). There is no divorce process, no inheritance, and no duties towards one another. The only two laws are that: 1. the man provides support for the child in case the woman is pregnant; 2. the woman waits for a period of 45 days before she enters into another Mut3a or. In (D) differences between RAC1B siRNA + pSMAD3-mut3A-transfected and TGF-β1-treated cells (red curve, tracing C) and RAC1B siRNA + vector-transfected TGF-β-treated cells (green curve, tracing B) are significant at 5:00 and all later time points (p < 0.05, Mann-Whitney U-test). Inset, the functionality of pSMAD3-mut3A and SIS3 in blocking the formation of pSMAD3-C after a 1-h treatment. None of the single inhA MUT2 (A16G mutation), inhA MUT3A (T8C mutation) and inhA MUT3B (T8 Amutation) band was seen . Isolated inhA gene mutation was found in 4/66 (6%) of MDR-TB strains and 6/21 (28%) of INH monoresistant strains. The turnaround time of line probe assay was 48 hours; whereas, it was 70 days for phenotypic DST (28 days for conventional culture growth and another 42 days for. if a muslim woman want to date a non muslim man.. is mut3a marriage necessary for her ? Click to expand... A Muslim woman cannot, must not, should not and will not marry a non Muslim man in any form of marriage, whether mut'ah or permanent marriage. If the man she loves is non Muslim, he would have to revert to Islam after extensive research and conviction to accept the faith. Reactions: Iron.
Bacteria deploy global programs of gene expression, including components of the SOS response, to counteract the cytotoxic and genotoxic effects of environmental DNA-damaging factors. Here we report that genetic damage promoted by hexavalent chromium elicited the SOS response in Bacillus subtilis, as evidenced by the induction of transcriptional uvrA-lacZ , recA-lacZ , and P recA-gfp fusions The goal of this study was to identify genes in Bacillus cereus , a bacterium commonly associated with plant seeds and roots, that are affected by compounds originating from a host plant, tomato, or another rhizosphere resident, Pseudomonas aureofaciens . We constructed a B. cereus chromosomal DNA library in a promoter-trap plasmid, pAD123, which contains a promoterless version of the green. diately 59 of the promoterless gfpmut3A construct in the promoter trap vector, pFPV25 (34). This clone was then electroporated into wild-type C. freundii and assessed for ﬂuorescence. It was determined that the rpsM::gfpmut3A plasmid gave rise to highly ﬂuorescent colonies (as visualized by ﬂuorescence microsco-py). This clone did not affect the growth rate, cell density, or ability of.
MUT3A -8: T8C: 6: MUT3B -8: T8A: 10: The need to implement sanitary guidelines so as to guarantee public health has been highlighted by an increase in MTBC infections concomitant with consumption of unpasteurized milk, home-made dairy products and food handling reported in the World . More importantly, lack of information on the TB history of all the cattle in our study raises concern on. Changing GG to AG (nifA1 mut3A) or AA (nifA1 mut3AA) increases transcription four- to fivefold in E. coli but only twofold in R. capsulatus. These results are consistent with the hypothesis that R. capsulatus can naturally transcribe −10 regions with a higher G+C content
In a high tuberculosis burdened country like India, rapid, cost-effective, and reliable diagnostic tools for tuberculosis are an urgent need of the hour to prevent inappropriate treatment strategies and further spread of resistance. This study aimed to estimate the proportion of new smear-positive tuberculosis cases with primary resistance to rifampicin and/or isoniazid as well as identify the. ΔWT2, MUT3A: 63 (91.3%) No change: WT: Table 3 . Patterns of amino acid changes and mutations on inhA gene. 3.5. Comparison of Genotype MTBDRplus Assay and Conventional Drug Susceptibility Testing for Identification of Drug-Resistant Isolates. Of total 62 rifampicin resistant M. tuberculosis isolates detected by conventional drug susceptibility testing, mutation in the rpoB gene was detected. gfpmut3a (8, 17) fused to a 645-bp PCR product containing the constitutively active promoter of the B. anthracis metalloprotease gene, inhA (BA1295). The PCR employed template DNA from B. anthracis 7702 and oligonucleotide prim-ers ES26 (5-ATTCTAGACCCAGGATTTTCACCCTTTT-3), containing an XbaI site (underlined), and ES44 (5-CGGGATCCTTCTTGATCCAATGCGT CAG-3), containing a BamHI site (underlined. mut3A-BMAL1 also stimulated CLOCK phosphorylation and (ii) mut3A-BMAL1 level was reduced by coexpression of CLOCK as. was observed for wild-type (WT) BMAL1 (Fig 1B). To explore contribution of. And while you had some people who would straight up say, yeah I know this is against the rules of the religion but I'm going to do it anyway, there were others who found some kind of excitement in finding a loophole to get away with doing things that were, Islamically speaking, not-so-kosher
@Ershad, I think it would be more constructive if you remove the part about Sunni view if you want to know why Shia allow it. If you want to know why Shia doesn't accept the Sunni argument that you mention in the post then you should make it more explicit and the answer is more or less what is implied by Gigili, i.e. Shia accept a different set of hadith from Sunni ip3_mut3A: undetectable: ND: ND: ND: ND: ND: Parameters are indicated for the different peptides analyzed in this study with the conserved domain of human ASF1A, ASF1AN (ASF1A 1-156), or human ASF1B, ASF1BN (ASF1B 1-156) as indicated in the first column. Open table in a new tab Epitope Tethering. The affinity of ip1_s for ASF1 remains modest compared with the nanomolar-range affinity of.
MUT3A MUT3B Biochemistry Results. Used Units: SI . SI units. Conventional units. Treatment. Start Date. Day 27. End Date. Day 196. Outcome. Completed. Regimen. Start Date End Date Reason For Ending H R S E Ofx Cm Am Km Z Lfx Mfx Pas Pto Cs Amx/Clv Mb Eto Dld Bdq Ipm/Cln Lzd Cfz Clr Ft AG/CP Trd Pa ART Cotrimoxazol Gfx Gfx 0.5 Action; Day 27 Day 88 Continuation of treatment: Day 88 Day 196. WT2 -8 MUT3A T8C Genotype MTBDRplus . A s s s on 1 90 2 93 1 90V 2 91P 3 97 3A 94A 3B Y 3C 94G 3D 94H gyrA Asp94Gly No rrs mutations embB M306V rrs WT probes codons MUT probes mutation WT1 1401-1402 rrs MUT 1 A1401G WT2 1484 rrs MUT 2 G1484T Genotype MTBDR sl ( second line) Type of mutations Local epidemiology Prior treatment history Source control Optimising dosing with site / co morbidities. For primers Mut3A/E3bio: same as Mut1A/E3bio. For primers Mut3B/D3bio: same as Mut1B/D3bio. For each mutation, two PCR fragment were amplified using Vent Exo‐ as a polymerase in order to obtain blunt ends, each fragment having one of the 5′‐ends blocked with a biotin tag. The fragments were purified by PAGE and subsequently on Streptavidin Paramagnetic Beads (Promega) according to the. Neonatal bacterial meningitis remains a disease with unacceptable rates of morbidity and mortality despite the availability of effective antimicrobial therapy. Citrobacter spp. cause neonatal meningitis but are unique in their frequent association with brain abscess formation. The pathogenesis of Citrobacter spp. causing meningitis and brain abscess is not well characterized; however, as with. Rationale: Conventional culture-based drug susceptibility testing (DST) for the second-line antituberculosis drugs is slow, leading to diagnostic delay with associated exacerbation of transmission, amplification of resistance, and increased mortality.. Objectives: To assess the diagnostic performance of the GenoType MTBDRsl line probe assay (LPA) for the rapid detection of mutations conferring.